Principles of gene manipulation

by R. W. Old

As in previous editions, the basic philosophy of this extremely popular text remains to present the principles and techniques of gene manipulation in sufficient detail for the non-specialist reader to understand them. However, in the five years since the last edition, molecular biologists have seen a dramatic increase in available techniques and applications. Every chapter in this fifth edition has been extensively revised or rewritten to take these developments into account - including a greatly expanded section on the polymerase chain reaction and its manifold applications; a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics; and extended coverage of gene transfer techniques. With entirely new illustrations in two colours throughout, this will remain the textbook of choice for undergraduates and postgraduates alike.